Prestressed steel columns are known as an efficient structural solution for great variety of temporary or permanent supporting systems for large span spatial frames and tensile surface structures. This work presents of full-scale three-dimensional tests carriedout for the assessment of structural behaviour of prestressed stayed steel columns. It was studied the effect prestress force level, stiffness of column braces and stays. Test setup and a new force measuring system for the column stays is fully described. Prestressed stayed steel columns have their strength dependant of parameters like: length, hollow section diameter, brace length and stiffness and axial stiffness of stays. Due to the complex behaviour of such columns that demands great computational effort for numerical simulations required for a parametric analysis it was used an experiment design tool coupled with neural network techniques employed to generate new data for the prestressed column buckling load. A study of the dynamic behaviour of prestressed columns using the finite element package ANSYS was carried-out in order to determine the column natural frequencies and their associated vibration modes. It was also studied the application of sudden loads to determine the dynamic amplification factor of this type of prestressed stayed steel column.