Over the past two decades, the emergence of an entrepreneurial economy based on innovation has generated new demands on society and set new tasks and roles for university research and metrology. Accordingly, on the one hand, as the research university becomes an enterprising university with government assistance, there are growing social and political demands for results. On the other hand, the metrology is required to evolve its concepts and metrics to meet the demands of the service sector and innovation processes which are dominant in the entrepreneurial economy. Thus, the result of this research is a methodology for impact assessment that may actually be implemented and may generate an important asset to PUC, as well as other research universities that seek to tread a similar path. And above all, this study provides answers to society. The author, as an entrepreneur and alumni of PUC, is motivated by the belief that it may contribute to a better characterization of the entrepreneurial university in the country by measuring the impact of entrepreneurship. Besides this thesis contributes to the emerging international literature on the subject, the work also brings an important empirical contribution to the country since there are no similar studies. This paper proposes a methodology for assessing the impact of entrepreneurship of research universities. It was developed from the analysis of the concept of entrepreneurial university, the theoretical framework on socioeconomic impacts of universities, transformations on their roles and on the dynamics caused by the evolution of an entrepreneurial economy, and in this context, concepts and emerging metrics of a metrology which is service-oriented and focused on innovation.