Within the framework of the Revision of the Urban Transportation Master Plan for the Rio de Janeiro Metropolitan Region (PDTU-2011) an innovative model was developed – first of its kind in Brazilian cities – for the estimation of truck flows within the study area. Productions and attractions vectors (P/A) of truck trip ends, as well as origin/destination (O/D) matrices for the 730 traffic analysis zones were estimated, and truck assignments for three daily periods (morning peak, afternoon peak, and inter-peaks) were conducted. Model methodology was based in another study conducted for the city of Seville (Spain). Two sets of P/A vectors were estimated, one for business-to-business deliveries (B2B) and another for household deliveries (B2H). Daily truck trip ends P/A vectors aggregated by city are presented, as well as a city level O/D inter-peak truck matrix and the results of its assignment to the PDTU-2011 simulation network. From those illustrative results, it is shown that only five cities (Rio de Janeiro, Duque de Caxias, Niterói, São Gonçalo e Nova Iguaçu) account for more than 80 per cent of truck trips produced and attracted within the Metropolitan Region, and that the most heavily used truck routes are comprised of BR-101, BR-116 and BR-040 expressways.