Motivation is one of the most studied constructs in general psychology in the scientific sphere worldwide. Understanding why a certain behavior occurs seems to instigate countless researchers who are focused on investigating its components. Likewise, identifying the motivational influences of social agents that determine the sports motivational climate, such as: coaches, parents and peers/teammates, seems to be critical in sports practice, especially from the athlete s perception in the context of high performance sport. Motivational climate can be defined as the environmental state of sports practice that influences motivation levels. Therefore, this research was structured from two empirical studies with the aim of developing, applying and validating a multidimensional measurement instrument for evaluating the motivational climate in high-performance athletes. In study 1, of qualitative design, 23 (n=23) high performance athletes, aged between 18 and 33 years, both genders, practitioners of 5 sports: judo, soccer, artistic swimming, Mixed Martial Arts (MMA) and beach volleyball were interviewed through focus groups, in a semi-structured format, to investigate the motivational influences of coaches, parents and peers/teammates on sports practice. Content analysis was performed using the Iramuteq software. From the results obtained in the qualitative portion and following the steps of instrument construction, content validation, judge analysis and semantic analysis, the Multidimensional Motivational Climate Questionnaire (MCMCM) was developed. In study 2, the new instrument was applied to a sample of 166 subjects (n=166), of both genders aged between 18 and 59 years, all high-performance athletes from various sports, individual and collective (soccer; futsal; basketball; rugby; martial arts). Psychometric analyzes were performed and the results indicate that the QMCM presents evidence of validity and reliability compatible with the purpose of this study. The aim of this questionnaire was to equip psychologists and sports professionals, increasing awareness of the importance of identifying and measuring the motivational climate in high-performance athletes.