Water, essential natural resource, is becoming increasingly scarce and unfit for human consumption. The biosorption is a technology that uses various types of biomass, including nonpathogenic microorganism suchs Rhodococcus rubber. This study aims at the removal of Co(II) and Ni(II) present in aqueous solutions using R. rubber. Microbiological studies, batch biosorption test were conducted to test the efficiency of biomass in heavy metals Co(II) and Ni(II) through the pH, biomass concentration and concentration of the metal. The reaction kinetics was studie and the isotherms made. Zeta potencial, infrared spectrometry and electron microscopy techniques were used for knowledge of the elements involved microscopy techniques were used for knowledge of the elements involved in the process biosorption. A 44 per cent removal of Co(II) and 36.6 per cent Ni(II) solution at pH 6 was obtained after 180 minutes of contact in a solution containing 3 g.L(-1) of biomass and 30 mg.L(-1) of metal solution. From the concentration of biomass 3 g.L(-1), there was a reduction in metal removal for both Co(II) and for Ni(II). The isotherm best fit in the current study was Freundlich for the two metals. The model of pseudo-second order was the best in the kinetic studies performed for the Co(II) and Ni(II). When considering the best parameters, therewere increased levels of large removal using one or two phases of new biomass in the solution. Treatment of biomass with NaOH was the most efficient removal rising to 97 per cent of Co(II) and 89.1 per cent Ni(II).