This study aims to evaluate healthcare professionals (HCP) during their multiprofessional postgraduate training - residency - and their perceptions of the care dispensed to transgender people in a university hospital in Rio de Janeiro city. It is highly important to value the transexuality subject from the HCP perspective in order to build proper professional education and permanent health education that allows experience exchange spaces between those professionals and the population. This work has as specific goals: 1) To identify the in-training HCP perception about the demands of transgender people who attend to the health facility, 2) To evaluate the intercommunication between HCPs and trans people regarding the resolution of their needs and 3) To evaluate the internalization and reification of gender dimension in the public health education during residency from the professionals perception. Gender discrimination episodes against people whose sexuality is considered deviating is recurrent in health care institutions.. For this work, we conducted 8 interviews with 2nd-year health care students during their multiprofessional residency. To perform data analysis the content analysis method was used to evaluate the narratives of the interviewees, using three categories: basic knowledge on transexuality and health needs, health care to trans people and interactions with HCP and gender and professional training. We observed that the difficulties in dealing with gender and sexuality topics, specially those regarding female sexuality, reduce the impact of the action of those health professionals. Our results evidentiate that the manner the HCPs deal with gender and sexuality and how they understand those subjects are barriers to transgender people on their access to health care services.