For the knowledge of the natural stress state of the soil, expressed by the coefficient of earth pressure at rest (k0), a laboratory test is used in undisturbed samples. The importance of the determination of this parameter for colluvium soils relates to the application in engineering works that involve buoyancy of earth and foundations. There are correlations in the literature for determination k0 that comes from researches developed considering sedimentary soils, typically from temperate or cold regions. In dealing with residual and colluvium soils, typical of tropical regions, it is not appropriate to use these correlations, since k0 is affected by several factors, such as degree of weathering, laterization processes and suction, among others that also affect saturated sedimentary soils. This research analyzes samples of a colluvium soil from the Pontifícia Universidade Católica do Rio de Janeiro (PUC-Rio). For the determination of the coefficient of earth pressure at rest of the unsaturated colluvium soil, a flexible wall equipment, with a system of suction control was used. In order to evaluate the influence of suction on the value of the coeficiente of erath pressure at rest, each sample was tested under different moisture contents: wetter, with suction of 10 kPa; intermediate with suction of 40 kPa; and drier with 100 kPa. The k0 values obtained in the tests ranged from 0,19 to 0,24. The coefficient of earth pressure at saturated colluvium (null suction) was also determined using Bishop and Wesley type, servo-controlled triaxial equipment. The value of k0 obtained for the colluvium soil in the saturated condition was equal to 0.36. The results in both trials followed, in part, the expected trend.