Aminoglycosides (AMG) belong to a class of antibiotics effective for the treatment of infections caused by Gram-negative and Gram-positive micro-organisms. Ototoxicity, nephrotoxicity and muscle disorders are side effects of using these substances, implying the importance of controlling the use of pharmaceutical formulations based on AMG with practical analytical methods for routine, sensitive and selective. The molecular structures of the AMG does not present any relevant chromophores groups that enable direct measurement of their optical activity, thus, the methods described in the literature for determining it normally to use of chemical derivatization implying high delays, high costs and toxicity (dice reagents used) in such methods. The studies present in this dissertation, it is proposed to use of gold nanoparticles (AuNPs) as analytical probes for spectrophotometric determination of AMG, by exploration of the resonance Plasmon effect on the surface of AuNPs. Was investigated the analytical potential of aqueous dispersions of AuNPs as probes for the determination of tobramycin (TBR) neomina (NEO), gentamicin (GENTA), kanamycin (CANA), streptomycin (EST) and amikacin (AMIC). Promising results were found for all AMG cited excerpt EST. In the case of TBR, an analytical method for its determination in ophthalmic solutions was proposed. The analytical curves were constructed by monitoring the transmitted light at 529 nm or 681 nm. The monitoring of the analytic signal in 529 nm yielding a linear range from 6.5 x 10-9 mol L-1 to 1.6 x 10-7 mol L-1 with good linearity (R2= 0.9943) and the limit of detection (LOD) equal to 6.2 x 10-9 mol L-1. The monitoring of the analytic signal in 681 nm yielding a linear range from 4.4 x 10-9 mol L-1 to 1.6 x 10-7 mol L-1 with good linearity (R2= 0.9949) and the LOD equal to 3.8 x 10-9 mol L-1. The method showed to be robust in a pH range between 2.6 and 4.5, for 120 min. The method showed satisfactory accuracy and demonstrated selectivity with respect to other AMG, EST, and with respect to excipients present in the samples analyzed. Yielded percentage recoveries for samples (simulated and real ophthalmic solutions) from 104.0 to 123.1 percent and from 101.1 to 123.6 percent, for the monitoring of the analytical signal at 529 nm and 681 nm, respectively. Using a comparative method to validate these results, it was found through the t-student test that the percentage recovery found through the AuNPs probes and the comparative method were statistically equal. Studies in saliva from patients under TBR treatment indicated the potential of the probe for the analysis of biological fluids.