Nowadays, the nanoparticles have been target of searches in several in various areas due its magnetic, photonic, optoelectronic and catalytic exclusive proprieties. The present work aimed to study the SERS response and the possible interaction between the gold nanoparticles and ethylenediamninetatraacetic acid and its chromium (III) complex. This complex was synthesized and characterized by elemental analyses ( atomic absorption spectrometry and CHN ), infrared spectroscopy, Raman spectroscopy and Ultraviolet-visible spectroscopy techniques. It was possible to see that, in this case, the ligand EDTA coordenates as a quinquidentate with the chromium íon, and one water molecule occupied the sixty valence position. In this paper was synthesized also a aqueous colloidal solution of gold nanoparticle by the physic method pulsed laser ablation. This method is very interesting because allow the formation of the particles without the action of another reagent, utilizing just gold and quarter. After that, its was characterized by ultraviolet-visible spectroscopy, SERS, SEM and TEM. The dates showed that the particles had relatively small diameters, near 10 to 30 nm and there was the presence of carbon monoxide molecules adsorbed in its surfaces, possibility from CO2 present in the air that is so much soluble in water and in some stage of ablation was reduced. In order that study and present the sensibility of the SERS techniques, solutions of EDTA and synthesized complex was prepared in such a manner as when its added inside the colloidal solutions its final concentration was 10(-5) mol / L. In order to analyzed by SERS, these mixtures was deposited in a glass plate that before was covered by a aluminum layer so as to extinct some interference from glass fluorescence. The SERS bands got was studied and we believe that the interaction occurred in the nanoparticles surface with a both ligand and complex was by hydrogen bond.