The present work begins with an investigation into the use of 3D Deep Learning models for enhanced strawberry detection in polytunnels. We focus on two main tasks: firstly, fruit detection, comparing the standard MaskRCNN with an adapted version that integrates depth information (MaskRCNN-D). Both models are capable of classifying strawberries based on their maturity (ripe, unripe) and health status (affected by disease or fungus). Secondly, we focus on identifying the widest region of strawberries, fulfilling a requirement for a spectrometer system capable of measuring their sugar content. In this task, we compare a contour-based algorithm with an enhanced version of the VGG-16 model. Our findings demonstrate that integrating depth data into the MaskRCNN-D results in up to a 13.7 percent improvement in mAP across various strawberry test sets, including simulated ones, emphasizing the model s effectiveness in both real-world and simulated agricultural scenarios. Furthermore, our end-to-end pipeline approach, which combines the fruit detection (MaskRCNN-D) and widest region identification models (enhanced VGG-16), shows a remarkably low localization error, achieving down to 11.3 pixels of RMSE in a 224 × 224 strawberry cropped image. Finally, we explore the challenge of enhancing the quality of the data readings from the spectrometer through automatic sensor positioning. To this end, we designed and trained a Deep Learning model with simulated data, capable of predicting the sensor accuracy based on a given image of the strawberry and the subsequent displacement of the sensor s position. Using this model, we calculate the gradient of the accuracy output with respect to the displacement input. This results in a vector indicating the direction and magnitude with which the sensor should be moved to improve the sensor signal accuracy. A Visual Servoing solution based on this vector provided a significant increase in the average sensor accuracy and improvement in consistency across new simulated iterations.