The increasing incidence of building vibration problems due to human activities led to a specific design criterion to be addressed in structural design. This was the main motivation for the development of a design methodology centred on the steel-concrete composite floors non-linear dynamic response submitted to loads due to human rhythmic activities. This way, the main objective of this work is to investigate the influence of steel-concrete interaction degree (from total to various levels of partial interaction) and the beam-to-column and beam-to-beam connections effect over the non-linear dynamic behaviour of composite floors. Thus, three dynamic loading models were utilized, in order to simulate human rhythmic activities such as jumping and aerobic gymnastics. The dynamic loads were obtained through experimental tests and based on international design codes and recommendations. Regarding the steel-concrete interaction degree, the stud and perfobond connectors are considered in this investigation. Even though this topic has been studied in the technical literature for nearly two centuries, the steel-concrete composite floors non-linear dynamic analysis submitted to human rhythmic activities has not yet been addressed so comprehensively, as far as the authors are concerned in this investigation, based on the consideration of the connectors (steel-concrete interaction degree) and connections (beam-to-column and beam-to-beam) effects. Considering all aspects mentioned before, the results have demonstrated that the design criteria should include the original nature of the dynamic excitation and, specially, the steel-concrete interaction degree and structural connections effects when the steel-concrete composite floors structural behaviour, related to the serviceability limit states (human comfort) are investigated.