The purpose of this work is to evaluate the behavior of 316L stainless steel, manufactured by Laser Directed Energy Deposition (L-DED) technique, that is, additive manufacturing, at SENAI Innovation in Manufacturing Systems and Laser Processing in Santa Catarina and sent to PUC-Rio in two different conditions: as manufactured (as built) and after heat treatment (as built + H.T.). Then, specimens of these two microstructural conditions were machined and tested under super long cyclic loading (VHCF) in the ultrasonic equipment located at the University’s Very High Cycle Fatigue Laboratory. The tests were performed with frequencies of 20 kHz, using two distinct stress amplitudes, 201 MPa (𝜎1) and 230 MPa (𝜎2), previously defined. Subsequently, some specimens were selected for fractographic analysis of their fracture surfaces by scanning electron microscopy. As for the results, it is considered that the fatigue-life of the as built condition was superior to that of the as built + H.T. condition, since its specimens reached the VHCF regime, even without having reached a run-out in all cases. In turn, the fractographic analysis indicated the presence of microstructural discontinuities in the specimens associated with defects (pores) in the fabrication of the materials. It was observed that the cracks nucleated on the surfaces of the specimens, not registering fish-eye formation, typical fractographic characteristic of loads in the VHCF regime.