The objective of this dissertation is to assess the potential of the Chemistry Department at PUC-Rio to develop and adopt Green Analytical Chemistry’s methods, within the perspective of contributing for the success of PUC-Rio’s Environmental Agenda. Within the institutional context in which this research is situated, it is assumed that its results will have a great potential for application in various departments of PUC-Rio, in particular in the Department of Chemistry. This research can be considered descriptive and applied and the methodology encompasses bibliographical and documental research, field research by mean of semi-structured interviews and case study. The main results can be summarized as follows: (i) survey of analytical methods that have been developed and used worldwide and have contributed to the advancement of Green Analytical Chemistry, (ii) green profiles of the laboratorial practices taught by General Chemistry Laboratory within the Chemistry Department at PUC-Rio; (iii) indication of laboratorial practices that can be improved or replaced by greener ones; (iv) a set of Green Analytical Chemistry’s actions to be considered in future revisions of PUC-Rio’s Environmental Agenda.