Título: | EVALUATION OF THE OIL CONTAMINATION OF THE ESTUARINE ENVIRONMENT OF THE GUANABARA BAY (RJ) BY THE FLUOROMETRIC DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN THE MUGIL LIZA FISH BILE | |||||||
Autor: |
ROBERTA LYRIO SANTOS NEVES |
|||||||
Colaborador(es): |
ROBERTA LOURENCO ZIOLLI - Orientador TEREZINHA FERREIRA DE OLIVEIRA - Orientador |
|||||||
Catalogação: | 17/JUL/2006 | Língua(s): | PORTUGUESE - BRAZIL |
|||||
Tipo: | TEXT | Subtipo: | THESIS | |||||
Notas: |
[pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio. [en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio. |
|||||||
Referência(s): |
[pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=8681&idi=1 [en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=8681&idi=2 |
|||||||
DOI: | https://doi.org/10.17771/PUCRio.acad.8681 | |||||||
Resumo: | ||||||||
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in
the
environment. The main source of contamination is
antropogenic, and oil spills are
one of the main PAHs sources for the aquatic environment.
This work evaluates
the usage of PAH metabolites in fish bile (Mugil liza) as
biomarkers in the aquatic
environment. For this evaluation two distinct areas were
monitores: Guanabara
Bay, RJ, known for its chronic oil contamination, and
Itaipu, Niterói, RJ, the
control area. Physico-chemical measurements: pH, dissolved
oxygen, conductivity
and transparency were made in situ and fish varying from
35 to 50 cm were
selected for sampling homogeneity and sexual maturity. In
the laboratory,
morphometric measures were taken and the fish bile was
extracted. After
optimizing the analytical method the samples were analysed
by diluting each bile
sample in ethanol 48% (1:2000 v/v) and fluorimetric
measurements were made in
excitation/emission wavelengths of 332 nm/383 nm. The mean
total PAHs
concentrations in the bile samples collected in the
Guanabara Bay were
significantly different from the control area, Itaipu. In
the Guanabara Bay the
means were 7,0 ± 3,4 (n=19) and 10,4 ± 6,4 (n=12) mg L-1
pyrene equivalents, in
winter and summer, respectively. In Itaipu, the mean HPA
concentration was 1,8
± 0,7 (n=11) mg L-1 pyrene equivalents. These results
indicate that this method
can differentiate contaminated areas from non contaminated
ones, making the fish
Mugil liza one possible biomonitor in the Guanabara Bay,
RJ. Additionally, this
analytical method has advantages compared to other methods
because it is less
time consuming and is inexpensive and therefore could be
used as a preliminary
monitoring tool.
|
||||||||