The present work aimed to evaluate the use of a polyamine-based biosurfactant for quartz flotation. For this work, characterization studies of the quartz and hematite minerals were conducted using X-ray diffraction (XRD) and infrared spectroscopy (FTIR). After the interaction between the biosurfactant and the minerals, a strong interaction between the biosurfactant and quartz and a weaker interaction between the biosurfactant and hematite were identified. The FTIR spectrum after interaction presented functional groups characteristic of the biosurfactant (NH2, CH2, C=O, and NH), which showed more intense peaks after interaction with quartz, indicating greater adsorption with quartz than with hematite. Zeta potential measurements indicated a possible electrostatic interaction between the biosurfactant and quartz, showing a charge reversal at pH 2.4 after the interaction. For hematite, there was a small shift in charge reversal, from pH 4 to pH 4.2, indicating a minor interaction, which suggests selectivity for quartz. Contact angle measurements showed greater hydrophobization of quartz compared to hematite, with the contact angle of quartz after hidrofobization changing from 33 degrees to 90 degrees, while the angle for hematite after hidrofobization changed from 45 degrees to 62 degrees. According to the results, the biosurfactant exhibited surfactant properties, reducing the surface tension from 70 to 40 mN/m with 40 mg/L of biosurfactant. After the characterization and interaction studies, microflotation studies of quartz and hematite with the -106 +44 micrometers fraction were conducted. The effects of pH (2-11) and biosurfactant concentration (5-100 mg/L) on mineral floatability were evaluated. In the case of quartz, an increase in pH and biosurfactant concentration favoured floatability, achieving 98 percent quartz flotation at pH 11 with a biosurfactant concentration of 30 mg/L. In relation to hematite, the behaviour was similar to that of quartz, with lower floatability, reaching 80 percent hematite flotation at pH 10 with a concentration of 100 mg/L. Two points were observed: above pH 10, hematite floatability decreased, and higher concentrations of BS were needed to float hematite, opening an important window for selectivity in mineral flotation systems. Finally, flotation studies of mineral systems (60 percent hematite and 40 percent quartz) were conducted to evaluate pH (2-11), biosurfactant concentration (5-100 mg/L), and depressant concentration (5-100 mg/L). The results indicated that an increase in pH favoured metallurgical recovery and quartz content, confirming the hypothesis of individual flotation. The increase in biosurfactant concentration also favoured metallurgical recovery and quartz content up to a certain concentration. The increase in depressant concentration also favoured metallurgical recovery and quartz content up to a certain concentration. The best metallurgical recovery and quartz content were achieved at pH 11, with a biosurfactant concentration of 40 mg/L and a depressant concentration of 50 mg/L.