Título: | GREEN METHOD FOR QUANTIFICATION OF LAVENDER AND SWEET ORANGE ESSENTIAL OILS IN BLENDS BY SYNCHRONOUS FLUORESCENCE FIRST DERIVATIVE | ||||||||||||
Autor: |
BEATRIZ SERRAO MONTEIRO BASTOS |
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Colaborador(es): |
RICARDO QUEIROZ AUCELIO - Orientador ROSANA CANDIDA MACEDO - Coorientador |
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Catalogação: | 24/OUT/2024 | Língua(s): | ENGLISH - UNITED STATES |
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Tipo: | TEXT | Subtipo: | THESIS | ||||||||||
Notas: |
[pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio. [en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio. |
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Referência(s): |
[pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=68466&idi=1 [en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=68466&idi=2 |
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DOI: | https://doi.org/10.17771/PUCRio.acad.68466 | ||||||||||||
Resumo: | |||||||||||||
A simple green analytical method for the quantification of sweet orange and
lavender essential oils (EOs) content in blends was developed using synchronous
fluorescence first derivative spectra and sample preparation by surfactant-free
microemulsions (SFMEs). Excitation and emission wavelengths pairs (Absorbance measurements in the excitation wavelength/Absorbance measurements in the emission wavelength)
were determined for both EOs (at 336/436 nm for sweet orange and at 330/388 nm
for lavender). Optimization was conducted to establish the condition to prepare the
SFMEs systems: 50 microlitres of oily phase containing EO diluted in octan-1-ol (1:4, v/v),
2.0 mL of water and propan-1-ol up to 5.0 mL final volume. Different proportions
of EO in the oily phase were prepared for analytical curves construction to monitor
curve s sensibility. Synchronous fluorescence measurements were performed, and
the acquired data were corrected by inner filter effect. Considering the first
derivative of the synchronous scanning, data were extracted at 316.8 nm (Stokes shift = 100
nm) and 352.0 nm (Stokes shift = 58 nm) for sweet orange and lavender EOs, respectively,
with determination coefficients above 0.99. Limits of quantification of 8.0 micrograms mL-1
and 65.8 micrograms mL-1 were obtained indicating that 0.46 percent of sweet orange and 3.69 percent
of lavender EOs can be quantified in mixtures. Simulated blends (25:75 percent, 50:50 percent
and 75:25 percent, volume proportions) were also evaluated with recoveries of 71.5 –
128.9 percent and coefficient of variation between 3.1 percent – 11.6 percent. Additionally, the
method s greenness was evaluated through three green metrics (Analytical Eco-Scale, GAPI, and AGREE) with aligned results.
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