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ETDs @PUC-Rio
Estatística
Título: OIL SPILLS AND HUMAN HEALTH - PAH METABOLITES IN URINE AS EXPOSURE TRACERS USING TANDEM MASS SPECTROMETRY
Autor: LIVIA ARAUJO LOREDO
Colaborador(es): CARLOS GERMAN MASSONE - Orientador
Catalogação: 29/AGO/2023 Língua(s): PORTUGUESE - BRAZIL
Tipo: TEXT Subtipo: THESIS
Notas: [pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio.
[en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio.
Referência(s): [pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=63830&idi=1
[en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=63830&idi=2
DOI: https://doi.org/10.17771/PUCRio.acad.63830
Resumo:
A major crude oil spill occurred off the coast of Brazil in 2019 affectinga 4,334 km stretch of coastline, 11 states and several localities. As a result,residents came into direct contact with crude oil and were exposed tocontamination by polycyclic aromatic hydrocarbons (PAHs). PAHs arecarcinogenic organic compounds of petrogenic and pyrogenic origin, whichare ubiquitous and persistent. When humans are exposed to thesecontaminants, the organism can metabolize and generate metabolites ofthese PAHs with probable negative effects on human health. Therefore,this work aimed to analyze the exposure of a fishing community (located inCa-navierias, BA) to PAHs from oil spills through the analysis of PAHmetabolites in urine. For this, the 44 samples were extracted by solid phaseextraction (SPE) and analyzed by liquid chromatography coupled to triplequadrupole mass spectrometer (HPLC-MS/MS). This work also includes thecomparison of different extraction methods in 18 selected urine samples.Solid phase extraction and liquid-liquid extraction were performed at theLeopoldo Américo Miguez de Mello Research, Development and InnovationCenter (CENPES), while SPE extraction was performed at PUC-Rio. Theresults for the 44 samples analyzed at PUC-Rio showed that 4 of the 11metabolites of interest were identified and quantified. For 1-OH-Nap theconcentration range was (less than LQ - 44 n mL(-1)), (less than LQ-21 ng mL(-1)) for 2-OH-Nap,(less than LQ -5.4 ng mL(-1)) for 3,9-OH-Phe and (less than LQ- 0.76 ng mL(-1)) for 6-OH-Chr.When comparing the results obtained with literature works, it was observedthat the residents of the community of Canavieiras had low concentrationvalues of the metabolites that were quantified. Even so, it is important theexistence of works focused on the monitoring of these compounds since notonly the environment but also present themselves as a risk to human health.
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