With the advent of the COVID-19 pandemic, numerous studies on the disease were initiated, a disease originated from a cytokine storm. Although vaccines are effective in combating the spread of COVID-19 and, more recently, the antiviral drug Paxlovid (Trade Mark) has been approved by ANVISA (Brazilian Health Regulatory Agency) for the treatment of COVID-19, studies on forms of treatment are still needed. Among the new therapeutic strategies, the use of gold nanoparticles (AuNPs) has been in the spotlight due to their potential antiinflammatory effect. This work aims to study the potential anti-inflammatory properties of AuNPs, synthesized by pulsed laser ablation (PLA) in water, in RAW 264.7 macrophages. Furthermore, the present work aimed to investigate the possible connection between the immunomodulatory effect of the nanoparticles and the secondary products obtained through the CO2 reduction reaction (CO2RR) activated by PLA in water. In this sense, we analyzed the proliferation, cytotoxicity, levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in RAW 264.7 macrophages subjected to treatment with AuNPs and exposed to lipopolysaccharide (LPS), which was used as a precursor agent of the inflammatory process. In conclusion, the results showed that the AuNPs at a concentration of 10 (micro)g/mL did not have a cytotoxic character and reduced ROS and RNS. AuNPs demonstrated promising anti-inflammatory activity with reduced cytotoxicity values, which points to future clinical applications.