Gender issues have always been a topic of great relevance within Psychology. The search for understanding this concept finds explanations in several theories. We can say that gender is associated with social categorization. Gender orientation concerns how people define themselves according to the gender roles they assume. Gender orientation boundaries can range from extreme agreement with the biological sex to extreme disagreement with the biological sex. Thus, considering gender orientation, it was possible to develop an instrument to measure this construct. The present work was divided into two studies. The first sought to elaborate and search for evidence of the validity of the Gender Orientation Scale based on the seven factors of human sexuality (OriGen Scale). The instrument presented two factors: Typified and Mixed. The Typified factor measures how much people consider that they represent and play roles in society as a typical person of their biological sex. The Mixed factor measures how much people consider that they represent and play roles in society of both possible biological sexes. In addition to a factor structure adjusted to the theoretical model, the instrument presented other satisfactory evidence of validity, such as relationships as expected with the big-five personality factors, gender roles, and adult attachment. The second study tested gender orientation and depression as predictors of suicide risk and investigated relationships between these variables and internalized homophobia. The negative correlations between suicide risk, the Typified factor of gender orientation and age; and the positive correlations between suicide risk, the Mixed factor of gender orientation, depression, and internalized homophobia are highlighted. A structural equation analysis was conducted with the variables the Typified factor of OriGen, the Mixed factor of OriGen, and internalized homophobia explaining depression, and depression explaining the risk of suicide. This model explained 8% of the variance in depression and 33.8% of the variance in suicide risk. Structural equation analyzes indicated that gender orientation does not significantly impact suicide risk. That is, depression and internalized homophobia are more explanatory of suicide risk than gender orientation. We concluded that this thesis contributes to Psychology as a starting point. A point for greater understanding of gender issues and their impact on the lives of individuals, regardless of which social group they belong.