Ultra-high Performance Concrete (UHPC) is an advanced cementitious material that has excellent mechanical performance, ductility and durability due to a high packing density and the use of fibers, contributing to increase the structures lifespan. Most of the structures are subject to cyclic loads, which vary with time, resulting in fatigue damage such as the formation and propagation of cracks that could compromise its integrity. Thus, it is essential to understand the behavior of materials subjected to fatigue so that safe and proper design guidelines can be proposed for the appropriate performance of the structures. Therefore, this work aims to investigate the behavior of pre-cracked ultra-high performance concrete under flexural fatigue, quantifying its mechanical deterioration during cyclic loading through both crack mouth opening displacement (CMOD) and stiffness, which will contribute to the study of this special type of concrete. Equations were proposed to predict fatigue life according to the upper load limit during the cyclic loading and to establish the endurance limit of ultra-high performance concrete in 75,3 percent, considering the lower limit load equal to 30 percent of the upper limit. Also, when evaluating the post-fatigue behavior of samples that did not fail over 1,000,000 cycles it was possible to identify that the cyclic loading did not change the performance of the samples under bending, which was due to the use of upper loads below the endurance limit.