The objective of this dissertation is to present a simulation in the software Aspen HYSYS (Registered trademark) v8.8 of a sugarcane-ethanol plant covering a cogeneration and an enzymatic hydrolysis system, applicable to bagasse final use, and associated to a microalgae-cultive plant for the production of third-generation biodiesel through transesterification route. Additionally, the effects of two variables - designation of bagasse to enzymatic hydrolysis (x1) and of ethanol to dehydration (x2) - were statistically evaluated by 3(2) central composite design over energetic and environmental performance parameters restricted to the plant s scope and being neglected contributions of the rural area. In a base scenario similar to Brazilian’s reality – coordinates (0, 50 per cent) –, it was observed that the integration provides a quantity of biodiesel (26.6 kg.t-1 of sugarcane) energetically significant, besides the supply of ethanol (62.2 kg.t-1 of sugarcane). In this case, the energetic demand of the microalgae-biodiesel section pointed to be similar the conventional ethanol plant consumption. The adjusted models were robust and precise (R2 higher than 0.998). The statistical assessments pointed that x1 affects quadratically the answers and x2 promotes null or mild effects. Stands out NER values - ratio between energetic contribution of products and inputs of the system - between 0.763 and 0.904, which were close to 1, and FER values - ratio between energetic contributions of products and fossil share of inputs - between 4.33 and 10.88 that were higher than the 3, which configured renewability to the system. Among the optimization of the answers, it was found the coordinate (92 per cent, 0) for the global exergetic efficiency. Finally, the association of the ethanol and biodiesel plants provided promising results for energetic efficiency, ratio between total delivered energy and demanded fossil energy, and increase in exergetic efficiency of the original ethanol plant.