With the growth of industries and competitiveness between markets, the urban freight transport has been considered fundamental for the economy, but the importance of their direct relationship to the lives of people in cities has resulted in more congestion in the central regions. It is therefore important to have knowledge about the movement of urban freight inside the Metropolitan Area of Rio de Janeiro - MARJ. This work try to estimate origin destination matrices (O-D) from flows observed on the network. These counts were done for two types of times considered important for reasons of traffic restrictions, which are the peak of the morning (7:00 - 8:00) and late peak (17:30 - 18:30). After doing a literature review on the issue, the work defines between the known methods the one that was considered most appropriate for the estimation of O-D matrix. Decided for the method developed by Nielsen (1998) termed as Single Path Matrix Estimation - SPME. The method was applied to the road network of the master plan of the urban transport in the Metropolitan Area of Rio de Janeiro (PDTU-RMRJ), using software TransCAD.