PCBs, PBDEs and OCPs were determined in samples of small cetacean, squid and fish from the Central-northern coast of Rio de Janeiro. This region has great importance for the Brazilian economy and comprises two distinct regions, which are Norte Fluminense and Região dos Lagos. The levels of all POPs were higher in small cetaceans, followed by fish and squid. PCB levels ranged from 12.54 to 5202.95 ng g-1 (wet wt). The main congeners found were PCB 138, 153 and 180. Hexachlorinated PCBs were the predominant congeners in cetaceans and pentachlorinanted PCBs were predominant in fish and squid. PBDEs were found only in cetaceans, except in muscles of P. blainvillei. PBDE concentrations ranged from 7.82 to 184.02 ng g-1. Contamination patterns suggest the previous use of Aroclor 1254, 1260 and Penta-BDE mixtures in Brazil. The main organochlorine pesticide found in this study was p,p-DDE, which presented levels in the range of 4.47 to 373.97 ng g-1. Significant correlations were found between the levels of POPs and morphometric variables of all the individuals. Finally, the analysis carbon and nitrogen stable isotopes was performed in order to assess trophic relationships, feeding preferences and differences on their habitat. These results contribute to extend the database on organic contamination in the Brazilian southeastern coast and in the Southern Hemisphere.