The consideration of transmission costs is essential for an isonomic economic valuation of generation projects due to the competition among different sources. The transmission costs tend to be greater for power plants located far from the load centers than for those that are closer. The transmission cost of each agent is calculated through a tariff, whose value depends on the point of connection and might reflect a locational signal of the transmission network s portion effectively used. The existing methodologies have limitations that greatly hinders the locational signal, therefore, is required a complementary charge known as postage stamp that can be quite significant when compared with the locational portion. The existing methodologies do not consider the multiple dispatch scenarios for allocation of the transmission costs, which is a very important issue and can greatly influence the allocation and create a cost recovery problem, especially in countries with a strong presence of hydroelectric plants. Another important issue to be considered in methodologies for allocation of transmission costs is the calculation of the transmission tariff varying in accordance with the load blocks, whose benefit is enable the consumers to respond to the transmission tariff s economic signal, increasing economic efficiency. This thesis presents a new methodology based on the AS scheme that incorporates the multiple dispatch scenarios and provides the calculation of the transmission tariff per load block. To illustrate the application of the proposed methodology, it was used a 3 bus test system and the Bolivian power system. The proposed methodology is compared with the following methods: (i) Average Participation Factors (APF); (ii) Long Run Marginal Cost (LRMC) and (iii) Original AS. The results showed that the use of the proposed methodology significantly improved the locational signal, reducing the need of the postage stamp portion of the transmission tariff.