Bacteria Rhodococcus opacus has the ability of turn hydrophobic the surface of apatite mineral due compounds present in their cell wall, then for evaluate this particular characteristic of the bacteria, was studied the influence of different parameters to try to understand the interaction between bacteria and mineral. The results of electrophoretic mobility of the bacteria and mineral showed an isoelectric point around 2.8 and 2.5 respectively; after of the bacteria and mineral interaction was observed a change in the zeta potential curve of the apatite mineral. The results showed that there is a greater affinity between the bacterial cell wall and the surface of apatite mineral at pH around 7. It was observed that an increasing in the initial concentration of bacteria is favorable to uptake (mg bacteria/ g mineral). The experimental data of adhesion were fitted of the Langmuir and Freundlich models. Freundlich model showed a suitable adjustment and was obtained constants n equal 1.111, 1.154, 1.198 to 293 K, 303 K, 313 K respectively. The results showed the positive influence in the uptake when there is an increase of temperature and interaction time. Was evaluated the pseudo-first order kinetic model and were obtained rate constants of 0.0228, 0.0353, 0.449 min-1 to 293 K, 303 K, 313 K respectively. The results showed an activation energy of 25.91 kJ / mol, suggesting the predominance of chemical interactions between the bacterial cell wall and mineral surface. The images of scanning electron microscope revealed the multilayer adhesion of bacteria onto apatite mineral. This study provides some understanding for the potential use of bacterial as bioreagent in flotation.