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Título: SYNTHESIS, CHARACTERIZATION AND EVALUATION OF DRUG DELIVERY SYSTEMS BASED ON MESOPOROUS SILICA FOR IMAGE IN NUCLEAR MEDICINE
Autor: LUCAS TORRES MIRANDA SA
Colaborador(es): MARIA ISABEL PAIS DA SILVA - Orientador
RALPH SANTOS-OLIVEIRA - Coorientador
Catalogação: 11/SET/2012 Língua(s): PORTUGUESE - BRAZIL
Tipo: TEXT Subtipo: THESIS
Notas: [pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio.
[en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio.
Referência(s): [pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=20339&idi=1
[en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=20339&idi=2
DOI: https://doi.org/10.17771/PUCRio.acad.20339
Resumo:
Cancer is the second major leading cause of death worldwide and is estimated till 2030, 27 million new cases and 17 million of deaths. So, the development of new drug delivery systems (DDS) that allows a bioactive agent distribution in its therapeutic site in the right dose, in a specific rate, leading lower toxicity, and that can be used for treatment and diagnosis of several diseases. Recently, mesoporous silicas arise as promising DDS or carriers for radiopharmaceuticals, because of its highly organized structure, high superficial area and tunable pore size and volumes. In this work, were synthesized three potential DDS or carriers for radiopharmaceuticals for imaging in nuclear medicine. SBA-15 and its association with hydroxyapatite (HAP) by two distinct methodologies: precursors addition during mesophase formation (SBA-15/HAP_1); and precursors impregnation in SBA-15 previously synthesized and calcined (SBA-15/HAP_2). The materials were labelled with tecnecium-99m radioisotope and its labeling methodology and biodistribution were evaluated. SBA-15 was also conjugated with the aptamer Anti-MUC1 for active delivery to tumoral cells. The materials were characterized by: inductively coupled plasma optical emission spectroscopy (ICP-OES); thermogravimetric analysis (TGA); nitrogen physisorption; x-ray diffraction (DRX); infrared (IR), transmission and scanning electronic microscopy (TEM and SEM); and laser diffraction. The labeling and biodistribution evaluation were performed by gamma-ray counter and gamma-camera respectively. The materials were synthesized and have shown peculiar characteristics. SBA-15 has highly ordered mesoporous structure with high superficial area. The materials impregnated with hydroxyapatite showed structural differences: In SBA-15/HAP_1, hydroxyapatite nanocrystals almost blocked the entire mesopores, while in SBA-15/HAP_2, there were a partially blocking of the mesopores and also presented hydroxyapatite nanocrystals aggregates. The tecnecium-99m labeling methodology of the materials showed an 8 hours stability. SBA-15 conjugation with Anti-MUC1 for active delivery has shown a better recognition by tumor cells.
Descrição: Arquivo:   
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CHAPTER 1 PDF    
CHAPTER 2 PDF    
CHAPTER 3 PDF    
CHAPTER 4 PDF    
CHAPTER 5 PDF    
CHAPTER 6 PDF    
CHAPTER 7 PDF    
CHAPTER 8 PDF    
REFERENCES PDF