The assessment of source and origin of hydrocarbons and stable isotopes in sediments from Grussaí and Almirante Câmara canyons is part of the HABITATS Project, which aim to characterize the environment of Campos Basin e a better understanding of the ecosystem dynamics. On this study several organic parameters were evaluate, such aliphatic and aromatic hydrocarbons, hopane, sterane and also the isotopic ratio of carbon and nitrogen. The aliphatic fraction over the canyons indicates the contribution of vascular land plants as well as residue oil products, possibly drilling non-aqueous fluids or similar material. The concentration distribution of hopanes or steranes is characteristic at all stations of recent presence of oil, which can be associated to natural oil seeps.The assessment of PAHs concentrations, assisted by diagnostic ratios suggests pyrogenic source contribution. In the winter campaign, is observed in Almirante Câmara canyon a predominance of biomass burning source, however, the isotopic ratio of carbon and nitrogen indicates marine source, which do not corroborate the information obtained by analysis of hydrocarbons. The large number of stations and parameters analyzed allow an amplified view for evaluation.