Título: | DEVELOPMENT OF ELECTROANALYTICAL METHODS FOR THE DETERMINATION OF SIBUTRAMINE IN PHARMACEUTICAL FORMULATIONS AND IN PROTEIN-RICH FOOD COMPLEMENTS | ||||||||||||||||||||||||||||||||||||
Autor: |
JULIANA MACHADO DE CARVALHO |
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Colaborador(es): |
RICARDO QUEIROZ AUCELIO - Orientador KATIA CHRISTINA LEANDRO ANTUNES - Coorientador |
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Catalogação: | 22/OUT/2009 | Língua(s): | PORTUGUESE - BRAZIL |
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Tipo: | TEXT | Subtipo: | THESIS | ||||||||||||||||||||||||||||||||||
Notas: |
[pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio. [en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio. |
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Referência(s): |
[pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=14472&idi=1 [en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=14472&idi=2 |
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DOI: | https://doi.org/10.17771/PUCRio.acad.14472 | ||||||||||||||||||||||||||||||||||||
Resumo: | |||||||||||||||||||||||||||||||||||||
Sibutramine hydrochloride is among the most prescribed satiety inducer
for obesity control and it has also been added, in a fraudulent way, in proteinrich
food complement for athletes. There is no analytical method for
sibutramine described in the official literature. The goal of this work is to
develop and validate electroanalytical methods for the determination of
sibutramine in pharmaceuticals and in protein-rich food complements.
Differential pulse voltammetry (DPV) and square wave voltammetry (SWV)
were the techniques employed. The influence of electrochemical parameters
was studied in order to select the best working conditions such as supporting
electrolyte (Mcllvaine buffer), pH (4.0), scan rate (40 mV s(-1) for DPV and 240
mV s(-1) for SWV) and pulse amplitude (50 mV). The pre-concentration of
sibutramine in the working electrode (mercury drop) was not possible. The
electrochemical behavior in the mercury electrode was also investigated using
cyclic voltammetry. The reduction of sibutramine is probably reversible and
involves of 1 proton and 1 electron. The peak potential of sibutramine is about -
100 mV (Ag/AgCl3 mol L(-1)). In the validation process, the linear range of the
analytical response (from the limit of quantification to 33.3 mg L-1, in both
methods), the limit of detection (0.6 for DPV and 0.4 mg L(-1) for SWV) and the
limit of quantification (1.8 for DPV and 1.1 mg L(-1) for SWV) were evaluated.
Satisfactory precision was achieved. Recovery tests produced results of 90.4%
for both methods. These results were compared with the ones achieved by
HPLC and no significant statistical difference was found between them.
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