ETDs @PUC-Rio
| Título: | PESTICIDES IN ORGANIC SOILS OF NOVA FRIBURGO RJ: APPLICATION OF THE ACHE INHIBITION ENZYMATIC METHODOLOGY AND STUDIES ON THE INFLUENCE OF SOIL FERTILITY PARAMETERS | ||||||||||||||||||||||||||||||||||||
| Autor: |
MONICA SANTANA VIANNA |
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| Colaborador(es): |
ROBERTA LOURENCO ZIOLLI - Orientador |
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| Catalogação: | 10/NOV/2008 | Língua(s): | PORTUGUESE - BRAZIL |
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| Tipo: | TEXT | Subtipo: | THESIS | ||||||||||||||||||||||||||||||||||
| Notas: |
[pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio. [en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio. |
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| Referência(s): |
[pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=12460&idi=1 [en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=12460&idi=2 |
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| DOI: | https://doi.org/10.17771/PUCRio.acad.12460 | ||||||||||||||||||||||||||||||||||||
| Resumo: | |||||||||||||||||||||||||||||||||||||
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The objective of this study was to develop an analytical
methodology for
the determination of metomyl in soils through the
inhibition of the enzime
acetylcolinesterase (AChE), also regarding the influence of
soil fertility
parameters. The soil samples were collected from
agricultural areas in the microbasin
of São Lourenço stream, in Nova Friburgo district, RJ, in
order to
contemplate different soil categories and the areas
agricultural management.
This area is one of the main olericole producers in Rio de
Janeiro state, with
records of intensive use of agrotoxics. A research on the
types of cultures and
agrotoxic use and their respective application methods was
conducted, through
interviews with laborers, producers and suppliers, in order
to acquaint ourselves
with the area. The extraction and enzymatic determination
methods were
optimized, indicating no need for clean-up, which renders
the procedure an easy
operation, with smaller analysis periods and reagent use.
The use of microplates
in the measurement of the enzymatic activity of AChE
permitted the use of
smaller amounts of enzyme and substrate (Acetilcoline),
reducing the analysis
periods. Comparisons between recently contaminated samples
and those stocked
for periods varying from 24h to 1 week, in different
temperature conditions (25ºC, 4ºC and -15ºC), indicated
that a higher sample stability and conservation
can be obtained at 4ºC, although this is still dependent on
the concentration of
this methylcarbamate and the matrix constituents. The
optimized methodology
was applied in the quantification of methomyl in soil
samples of the area
successfully, indicating that it can be used in
environmental evaluation diagnosis
and in anticollinergic pesticides (carbamate and/or
organophosphates)
monitoring programs. The availability of practical and
inexpensive
methodologies helps controlling government organs in the
effective
implementation of routine analysis s that aim to subsidiate
government decisiontakings
and the elaboration of certificates for phyto-sanitary
control.
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