Título: | IN SITU ATOMIC FORCE MICROSCOPY AND MICROHARDENESS OF DENTIN THROUGHOUT THE CHELATION PROCESS | |||||||
Autor: |
GUSTAVO ANDRE DE DEUS CARNEIRO VIANNA |
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Colaborador(es): |
SIDNEI PACIORNIK - Orientador MARCOS HENRIQUE DE PINHO MAURICIO - Coorientador |
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Catalogação: | 21/MAR/2005 | Língua(s): | PORTUGUESE - BRAZIL |
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Tipo: | TEXT | Subtipo: | THESIS | |||||
Notas: |
[pt] Todos os dados constantes dos documentos são de inteira responsabilidade de seus autores. Os dados utilizados nas descrições dos documentos estão em conformidade com os sistemas da administração da PUC-Rio. [en] All data contained in the documents are the sole responsibility of the authors. The data used in the descriptions of the documents are in conformity with the systems of the administration of PUC-Rio. |
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Referência(s): |
[pt] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=6143&idi=1 [en] https://www.maxwell.vrac.puc-rio.br/projetosEspeciais/ETDs/consultas/conteudo.php?strSecao=resultado&nrSeq=6143&idi=2 |
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DOI: | https://doi.org/10.17771/PUCRio.acad.6143 | |||||||
Resumo: | ||||||||
Instrumentation methods currently used in endodontic
therapy create a
residual layer composed of organic and inorganic material
that frequently retains
bacteria and their subproducts. The available methods to
remove this smear
layer include chemical etching, ultrasonic and laser
techniques but none of these
is completely efficient and there is no universal
consensus regarding this subject.
In the present study the effect of EDTA, EDTAC and citric
acid upon radicular
dentin was analyzed by two methods. Dentin microhardness
was measured
before and after etching. Atomic force microscopy was used
to map and
characterize dentinary microstructural changes throughout
the chelation process.
Real time, in situ, observations of the process were
performed through the use of
a special sample holder - a liquid cell - that allowed the
observation of the
samples surfaces during etching. Image sequences were
acquired and an image
processing and analysis procedure was developed to
evaluate, both qualitatively
and quantitatively, the effect of the 3 chelators. The
development of this
methodology employing in situ microscopy and digital image
analysis is one of
the main contributions of the present dissertation.
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