The present work investigated the pressure drop generated by the flow through an annular configuration with rotation of the inner cylinder. The motivation for the work comes from the several industrial application of the configuration, such as chemical reactors, filters and in drilling operations for oil and gas wells. The experimental study was conducted in a specially designed test section forming an annular space with radius ratio of 0.65 and a length equivalent to 265 hydraulic diameters, what guaranteed the hydrodynamic development of the flow for the laminar and turbulent regimes. The study investigated the pressure drop along the annular space as a function of the flow rate, given by the Reynolds number, and of the rotation of the inner cylinder, given by the rotational Reynolds number. The range of Reynolds number investigated was from 260 to 10,000, while the rotational Reynolds number was varied from 60 to 1,000. Two fluids were employed in the testes; water and a mixture of water and glycerol. The pressure drop results were presented in the form of dimensionless friction factors. Preliminary tests for the case of no rotation of the inner cylinder were compared with results available in the literature, in order to validate the test section and the experimental procedure. The results displayed good agreement with the literature for both laminar and turbulent regimes. For the low Reynolds number test cases, the results obtained have shown that the inner cylinder rotation induces a peak in the distribution of the ratio of friction factor to the friction factor without rotation. The peak, of about 15%, is found for Reynolds numbers of the order of 500.