Organic compounds observed in interplanetary space bodies are of particular relevance for revealing the physical-chemical processes that may be related to life s origin. Amino acids, such as alanine, are supposed to have played a crucial role in this process since they are basic components of proteins, essential constituents of all living organisms. Information about their radioresistance is essential for the development of models on how prebiotics molecules are formed or resist travelling in the Solar System and beyond; in particular the destruction cross-section by electron impact needs to be known and the radiolysis process needs to be understood. The current study aims to determine the dependence of alanine destruction cross-section on electron beam impact with energies in the range of 100 eV - 1 keV for thickness targets range of 10 nm - 1000 nm. The sample s degradation by the electron beam was monitored by infrared spectroscopy (FTIR). Moreover, simulations performed by Monte Carlo method for alanine irradiation with electron beam energies from 250 eV up to 5 keV are presented. It is inferred by the CASINO simulations, that the considered parameters vary as a function of E(1.7). Experimental results indicate that the effective destruction cross section decreases exponentially with the sample s thickness until it reaches a saturation level. The effective destruction cross section increases with incident energy according to the power law of E (1.76). The bands A-values were determined by infrared spectroscopy method and are in the order of 10(-18) cm(2)/molec. A moderate qualitative agreement was found between modeling results and the experimental data. It is concluded that it will be significantly improved if the sputtering process is added to the analyzed radiolysis.