The fluoroquinolones are an important class of synthetic antimicrobial agents clinically used for over 30 years. In addition to antibacterial activity, some fluoroquinolones, as well as their metal complexes, presented cytotoxic activity and are therefore promising as antitumor agents. In order to obtain new complexes of Au (III) and other metal ions that have biological activity, this work had the objective to synthesize new complexes using ligands of the group of fluoroquinolones. These new complexes were characterized by various techniques such as elemental analysis, conductometry, infrared spectroscopy, electron paramagnetic resonance (EPR), nuclear magnetic resonance (NMR) of 1H and 13C NMR, UV-Vis, stead state and time-resolved fluorimetry studies. Biological activities were analized for all complexes. Usually, the fluoroquinolones coordinate to the metal ions as bidentate through the carbonyl and carboxylic acid ketonic carbonyl group. This was confirmed Cu(II) complex, however, for Au(III) and Bi (III) complexes, the results of IR and 1H and 13C NMR showed that the coordination was trough the nitrogen using the piperazine ring, a coordination not usually found for fluoroquinolones. The complexes of Au(III) shown to be active against the A20 tumor cell lines (murine lymphoma), B16-F10 (murine melanoma) and K562 (human myeloid leukemia). Complexes of Cu(II) showed significant antiparasitic activity, whereas complexes of Bi(III) have shown antibacterial activity.