Most laboratories do not have a computerized system for management procedures. The administration and control of the samples are made manualy through many forms of data sheets which are filled from the beginning, when the samples of biological materials are gathered at the hospital, up to the final identification at the laboratory. In this context, the organization of the information become very limited, while the information writting by hands and stored in books, its almost impossible to extract useful knowledge, which could help not only supporting decisions but also in the formulations of simples statistics. This thesis had two objectives. The development of a web system called BCIWeb (Bacterial Classifiation and Identification for Web) that could assist in bacterial identification and provide the technology necessary for the administration and control of clinical specimen coming from the hospitals and the discovery of knowledge in database system, through data mining methods using SOM (Self Organizing Maps) and Multilayer Perceptron Neural Networks (MLP) for classification and identificatin of bactéria. From the development of this friendly tool, in the case study, the historical data from LDCIC (Laboratório de Difteria e Corinebactérias de Importância Clínica) of UERJ Biology Department were entered into the system. The proposed intelligent methods for classification and identification of bacteria were analysed and showed promising results.