The Pantanal in Mato Grosso do Sul, in Corumbá city, possesses the Escola das Águas (School of Waters), so called since they are located in an area that is difficult access because of the influence of the Pantanal water cycle. A period noted by rivers flooding from December to June and the low waters in the remaining months. The purpose of this thesis is to analyze the children s ludic culture in a Escola das Águas and its relationship with the school experience, with the primary question of how the children s ludic culture is comprised. What do Pantanal children do for play and how do they play being isolated during temporary occasions from the urban environment. How do they learn, and which games they play? Do they have access to media? How do they express their play culture and learning of play in the school environment? How do they relate ludic culture and school culture in the period in which they are in school? This thesis will include observations, interviews, drawings produced by the children about games and photographs of games made by the children in Escola Fazenda Santa Mônica. Data survey lasted 15 days in August 2016.Thirty-one students, four teachers and two monitors have participated in the study. The data was analyzed and categorized into five thematic axes: 1. Type of game / game used; 2. Children s playful preferences 3. Ludic cultures means of transmission; 4. Presence of media in the ludic culture; And 5. Ludic culture and school culture. The research pointed out that the school culture is a limiting element of the children s ludic culture, especially when it comes to games taking place in nature. Pantaneira s culture is almost non-existent in the game activities developed by children.