Background Dependency Injection (DI) is a commonly applied mechanism to decouple classes from their dependencies in order to provide better modularization of software. In the context of Java, the availability of a DI specification and popular frameworks, such as Spring, facilitate DI usage in software projects. However, bad DI implementation practices can have negative consequences, such as increasing coupling, hindering the achievement of DI s main goal. Even though the literature suggests the existence of DI anti-patterns, there is no detailed documentation of such bad practices. Moreover, there is no evidence on their occurrence and perceived usefulness from the developer s point of view. Aims Our goal is to review the reported DI anti-patterns in order to analyze their completeness and to propose and evaluate a novel catalog of Java DI anti-patterns. Method We propose a catalog containing 12 Java DI anti-patterns. We selected 4 opensource and 2 closed-source software projects that adopt a DI framework and developed a tool to statically analyze the occurrence of the candidate DI anti-patterns within their source code. Also, we conducted a survey through face to face interviews with three experienced developers that regularly apply DI. We extended the survey in order to gather the perception of a set of 15 expert and novice developers through an online questionnaire. Results At least 9 different DI anti-patterns appeared frequently in the analyzed projects. In addition, the feedback received from the developers confirmed the relevance of the catalog. Besides, the respondents expressed their willingness to refactor instances of anti-patterns from source code. Conclusions The catalog contains Java DI anti-patterns that occur in practice and are useful. Sharing it with practitioners may help them to avoid such anti-patterns.