Gender relations and sexualities emerge in the school context constantly, in the bodies of students and teachers, in dialogues woven inside and outside the classrooms. Empowered by perceptions involving prejudices and discriminations, gender identities and sexualities, they inhabit the school spaces between tensions and resistances, seeking to build experiences in the midst of heteronormative regulations. In order to investigate gender relations and sexualities in school, we carried out a research based on the question: How are gender identities and sexualities understood in a school with a high level of intolerance to gender and sexual diversity and in a school with low rate of intolerance on the part of the students? To do this we conducted interviews with teachers from two municipal public schools, selected from a broader survey, where we used questionnaires with seventh-year primary school students in ten municipal schools located in the southern zone of the city of Rio de Janeiro. Thus, we intended from the contact with students and teachers, understand how gender identities and sexualities are perceived in school everyday. Starting from the theoretical reference of Michel Foucault, Joan Scott and Judith Butler on genres and sexualities, we construct our analytical gaze by problematizing the convergences and distances present in interviews and questionnaires. With this, we come to some final considerations: (1) there are differences in the way girls and boys perceive sexual diversity and gender; (2) girls tend to have a more tolerant view of gender and sexuality; (3) boys present a more prejudiced perception and accept heteronormative gender stereotypes and reproduction of lgbtfobia; (4) there are spaces for problematization of gender stereotypes and sexualities in the teaching perspective; (5) we find teachers who conduct discussions about gender and sexuality in schools and affirm the importance of these themes for the school curriculum.