The present work has as objective the evaluation of the effect of the roughness measurement on the mass measurement values of F2 class mass standards. The interest is analysing the conformity to the requirements of the International Recommendation R 111 of the International Organization of Legal Metrology (OIML) and to standards ISO/97, referring to the subject. The roughness concept and the parameters, roughness average (Ra.), and average of the roughness (Rz.), were adequately characterized in the context of the developed research. The roughness measurements were carried out with the following F2 class mass standards: 0.02 g, 0.05 g, 0.1 g, 0.2 g, 0.5 g, 1 g, 2 g, 5 g, 10 g, 20 g, 50 g, 100 g, 200 g and 500 g, with the aid of a profilometer SJ 400. The conventional method was used to remove small amounts of substance of the measured object. The used F2 class mass standards, 0.05 g, 0.1g, 0.2 g, 0.5 g, 1 g, 2 g and 5 g, were calibrated before and after the measurements, because they presented the smallest permited maximum errors. The statistical analysis related to the experiments is shown next. Finally, the measurement uncertainty of the experimental results of the standard F2 mass collection was calculated and the conclusions of this study were presented.