The mechanical agitation or air injection generate bubbles in the flotation cell; they are stabilized by the use of frothers. Hydrophobic particles adhere to the bubbles and by ascending movement reaches the surface of the cell forming the mineralized froth that leaves the cell by overflow and/or mechanical removal. The flotation froths are of great importance, especially with respect to the size of the bubbles and their stability, as well as mobility; these factors are crucial in the kinetics viability of the process, in the global recovery and in the grade of the concentrate. The frothers, their actions and properties have been studied, however during the last years the technology of flotation reagents has had important innovations and developments. Like other reagents, the frothers were also influences by biotechnology, leading to the introduction of a new class of frothers called biofrothers. The bacteria in their cellular walls produce a range of proteins and polysaccharides, whence by specific procedures it can be removed bioreagents which feature surfactants characteristics similar to synthetic reagents used in flotation. Despite of several investigations on frothers, there is still a large gap in regard to biofrothers. On this work was studied the morphology of flotation froths using biosurfactants produced by the bacteria Rhodococcus opacus and their metabolic byproducts and the investigations focused on studies of analysis of bubbles size, height of foam, stability and persistence of foam, surface tension, superficial velocity of gas; using different techniques and equipment such as Bubble Sizer (Anglo Platinum), Tensiometer K10T (Kruss), Bikermans Method. The evaluated variables were solution pH, frother concentration, air flowrate and time.