Currently, thousands of children are conceived around the world through assisted reproductive treatments (ART). These have brought about as a consequence the considerable increase of multiple pregnancies. Considering the relevance attributed by psychoanalysis to the experience of motherhood and the mother-baby relationship due to its implications on the maternal psyche and on the psychic development of the baby, multiple pregnancy conceived through ART reveals a distinct experience of the experience of motherhood classically Studied by psychoanalysts. For this reason, the author of this thesis sought to analyze, through a case study of a mother who conceived a pregnancy triplets through ART, the experience of motherhood crossed by these peculiarities, as well as the psychic and emotional development of each of the triplets, in the first two years of their lives. Therefore, an observation of infants was performed by Esther Bick method, conciled with an interview conducted when the babies were one year old. In the analysis presented, issues peculiar to the case were explored, such as the outsourcing of care found, making the understanding of the bond established between the mother and her babies a challenge for the author. Consequently, the researcher sought to understand how the babies dealt with maternal absence. Elements related to the phenomenon of generational transmission were identified differently with each of the babies. It was observed how the maternal bond and the innate constitution of each of the babies crossed the process of subjective constitution of each one of the babies, being discussed individually. It was sought to propose a reflection that could be extended to other cases of twin motherhood, about how this experience is experienced by the mother of twins and how the twin brothers can build their psychic life.