Nowadays, the constant technological advances, and consequently the graphical user interfaces, have become more and more present in the humanmachine interaction. However, in a context where intelligent systems, such as robotic systems, are already a reality, there are still gaps to be filled when we think about integrating robots with custom and complex activities. This research aims on the analysis of Robot User Interfaces (RUIs) usability. When well executed, such interfaces allow operators to remotely perform tasks in complex environments. To that intent, our hypothesis is that, if RUIs are conceived considering the specificities of these interaction models, operational failures will be reduced. The main goal of this research was to evaluate specific guidelines for robotic systems, understanding their relevance in usability. For a theoretical basis, the existing models of interaction with robots and autonomous systems were raised; as well as the design principles that apply to these models. After a bibliographic review, we conducted contextual interviews with users of robotic systems, and usability tests to reproduce, in interfaces with and without RUI guidelines, the interaction processes in the task completion. The final results of the applied techniques proved the validity of the hypothesis, as the systems developed with interfaces specific to the interaction with robots provided better usability and mitigated the occurrence of array of human faults.