This work is an effort in the Psycholinguistics area and it is interfaced with that of Cognitive Translation Studies. It examines the process of textual segmentation of subtitles in the research on translation expertise in subtitling, an under investigated Audiovisual Translation mode in this regard. We consider, especially, the textual segmentation between lines of the same subtitle, a highly specific and indispensable skill that allow subtitlers to render their work in a way that optimizes the processing under these conditions. Based on studies of Expertise in Translation (Shreve, 2006) and language processing with focus on the Garden-Path Theory (Frazier e Fodor, 1978), we designed two experiments involving novice and experienced translators to evaluate the performance of both groups on the textual segmentation process (experiment 1) and segmentations revision (experiment 2); in both cases subtitles were in Portuguese. Experimental paradigms with high ecological validity, where the tasks were comprised of actual phases of professional work, were performed in a specific subtitling software. The results of experiment 1 showed superior performance of the professional group, which was confirmed in experiment 2, favoring the hypothesis of the authors that i) the experience is a relevant factor in the subtitle segmentation process; ii) experienced translators are sensitive to segmentations that can compromise reading (like garden path sentences). The preliminary characterization of expertise in subtitling and the new data added to the discussion on TGP are the main contributions this work achieves.