The growing world population, coupled with changes in lifestyle, result in an increasing demand for food, feed and energy crops. Brazil is increasingly producing and supplying these crops for other parts of the world. The Cerrado has become the centre of Brazil s soybean industry. The natural savannah has been replaced by crop monocultures which are associated with intensive use of synthetic fertilizers and pesticides. This study determines to which extent the application of agrochemicals in the cultivation of soybean contributes to the pollution of local river basins in the Cerrado. As a measure to quantify this impact, the grey water footprint (GWF) of soybean cultivation in a typical farm in the municipality of Correntina-BA is calculated for 5 cropping years. The most significant pollutant for all years was the pesticide 2,4-D. The GWF of soybean cultivation for the case study in the period ranged from 7,661 to 13,587 m3 per hectare and 2,441 to 7,651 m3 per tonne of soybean. The average water pollution level (WPL) associated with the production of this crop at river basin level was 48.6 percent. The average water pollution level (WPL) associated with the production of this crop at river basin level was 48.6 percent with values ranging from 36 percent to 83 percent. The calculated GWFs and WPLs show a large variation among different cropping seasons. The GWF in 2013/2014 had discrepant values, being 43.6 percent higher than the value in 2010/2011. This difference is mainly due to a higher application of the pesticide, from 0.80 kg/ha to 1.42 kg/ha. The WPL in 2013/2014 reached 83 percent . The results indicate that following the local trend of further intensification of large scale agriculture, the pollution of local water bodies with dissolved agrochemicals will increase to the point that it is likely to soon violate the local water quality standards.