Immunoassays in biological samples are based on methods that quantify the antigen-antibody link using a magnetic marker attached to the antibody. Recently, magnetic methods of detection have been applied using markers with magnetic nanoparticles in its interior. This can lead to early diagnosis of certain pathology such as tumors, autoimmune diseases etc. With this objective, built a magnetometer to perform this characterization. We use a tri-axial Hall probe, an electromagnet powered by a bipolar current source and a accurate positioner system. All components of the magnetometer were controlled using Lab View language. The interface with the user is extremely versatile and is made through a text file where any sequence of magnetic fields (from 0,2 mT to 1 T) to be applied to the sample can be specified. The performance of the built magnetometer was compared with a commercial SQUID magnetometer and an mean squared error of 0.43% was found in the magnetization of the particles of nickel for magnetic moments in the order of 10-4 Am2. Finally, as an example of application, nanoparticles of cobalt ferrite with magnetic moments in the order of 10-5 Am2 were coated with various surfactant and its characterization was performed using the built magnetometer.