Translate the Penn State Worry Questionnaire (PSWQ) to Portuguese and evaluate the psychometric properties and the latent structure of the translated version in a large Brazilian college student sample to determine its psychometric reliability and validity. A first study employed 871 undergraduate students from different Universities of Rio de Janeiro ranging from 17 to 68 years of age. An exploratory factor analysis (EFA) was employed to analyze its latent structure of 16 items that composes the scale. A second study employed 978 college students with the same characteristics from Study 1. A confirmatory factor analysis (CFA) was used to investigate eventual factorial artifacts of the scale previously described in the literature. Results indicated that PSWQ had an adequate internal consistency. In the first study, the EFA yields a two-factor solution. One factor was related to worry presence and incorporated all the 11 positive-worded items stated in a non-reversed form. The other was associated to worry absence and incorporated all the five negative- worded items stated in a reversed form. In a second study, the CFA leads to a three- factor solution. One factor incorporated all the PSWQ items whereas the two other factors were linked to the reversed and non-reversed items. Correlations coefficients of these two reversed and non-reversed factors with the total scores of the PSWQ and the trait subscale of the State-Trait Anxiety Inventory suggest that the factor composed by the five items do not contribute in a meaningful way to the PSWQ latent structure. Psychometric evaluation of the Portuguese version of the PSWQ indicated that the scale can reliably measure the presence of the worry construct in a non-clinical sample. Analysis of its factorial structure suggested that the worry absence factor might be a methodological artifact due to a wording effect of the reversed items.