This thesis has as its object the Women s High School of the Associação Feminina Beneficente Instructiva de São Paulo, AFBI, created by Anália Franco. A study on the teacher training course that aimed to send them to the nursery schools founded by the Association in the city and countryside of São Paulo, in the first decade of the 20th century. The research analyzed Anália Franco s trajectory as a teacher and director of AFBI, the course, the program, aspects related to teacher training for nursery schools, identifying the objectives, advances and difficulties encountered by Anália Franco in maintaining the course. The time frame adopted is the period that extends from 1902 to 1917 according to the sources found, which allowed us to relate the course to a promising moment of implementation of several instructive reforms directly related to the republican ideals of civility and regeneration. The methodology used was the documentary analysis of different sources: the periodicals found in the digital collection of the Hemeroteca of the National Library, the magazines Album das Meninas and A Voz Maternal published by Anália Franco, the Manual for teachers and the Manual for mothers, in addition to some AFBI reports, digitized on the Carlos Chagas Foundation website. Also, part of the analyzed documentation were the Teaching Yearbooks found in the São Paulo State Archive and the out-of-print memoir of a former student of the Association, published in 1959, in addition to the academic productions, the theses and dissertations about the educator and their association that preceded this thesis. Through a cross-analysis of the sources consulted, it was possible to verify that, with the Liceu, Anália Franco aimed to serve nursery homes with teachers trained for the early childhood segment. She faced economic problems, related to the subsidy for the work, the low vocational interest of the asylum seekers in becoming teachers, the difficulty in pedagogical materials for the course, in addition to students who needed to be literate during the course, factors that hindered the demand for urgent training to compose the teaching staff for the nursery schools and which made her create solution strategies, prioritizing another approach other than technical training, initially planned. An advertisement calling for interested candidates to be substitute teachers was the indication of a solution we found in the research, indicating that training would be through guidelines and manuals. Through research, we located some former students who completed the course and were teachers in the AFBI school network. Mapping part of the educational work of educator Anália Franco, focusing the research on the pedagogical training of teachers for the AFBI nursery schools, can constitute a contribution to studies on the teaching professionalization of a special professional body – primary teaching and nursery schools, study relevant to the field of history of education, especially to the history of the teaching profession.