Hydroxyapatite is among the ceramic biomaterials of greatest interest in the medical field, because, in addition to its similarity with the inorganic phase of bones and teeth, it presents excellent biocompatibility and bioactivity. Due to its wide application and the expensive surgical processes, it is necessary to develop more viable processes and materials with better properties, therefore, the work aimed the hydroxyapatite synthesis (HAp) by precipitation method using hen’s eggshells as a calcium source in the presence of different sucrose concentrations, to evaluate its effect on the characteristics of the synthesized materials. A comparative study was also carried out with HAp samples obtained without sucrose and with commercial Ca(OH)2. The use of eggshells appears as an attractive raw material since it is mainly composed of calcium carbonate and because it is an abundant and cheap material. The eggshell was calcined at 1000 Celsius to obtain CaO, which was hydrolyzed to obtain Ca(OH)2. The materials were obtained by the precipitation reaction between Ca(OH)2 and H3PO4 at room temperature with pH control, using different amounts of sucrose. The obtained materials were characterized by X-Ray Diffraction (XRD), Scanning Electron Microscopy with field emission (SEM-FEG), N2 to 77K adsorption (BET), Transmission Electron Microscopy (TEM), infrared spectroscopy with Fourier transform (FTIR), thermogravimetric analysis (TG) and X-Ray Fluorescence (XRF). The XRD results confirmed the formation of HAp. The FTIR showed the bands corresponding to HAp, corroborating the results of DRX, in addition to the presence of carbonate bands. The results of EDXS confirmed the composition of the samples, being of P, O and Ca. The SEM characterization allowed the evaluation of the morphology changes for the different amounts of the sucrose used, forming spheroidal particles. The BET results showed an increase in specific surface area with the rise in the sucrose amount, from 36 to 93 m2/g, due to the rise in porosity and decrease in particle size, a result confirmed by TEM, with particle size changing from 50 nm to values around 10-20 nm. The resazurin reduction assay was performed for the 24h period to evaluate of cytotoxicity. The results obtained indicate that the samples were non-cytotoxic. The bioactivity assay was carried out in McCoy medium for 3 and 7 days, showing dissolution results of the samples compatible with characteristics of bioactive surfaces. The formation of the bone-like apatite layer was not observed for the analysis period. The synthesized materials showed properties similar to those obtained from synthetic calcium sources. Therefore, it presents promising potential in bone tissue engineering applications, besides contributing to the recycling of this bio-residue, the eggshell. The high specific surface area and small particle size results obtained indicate that sucrose can be a promising additive/template that is easy to obtain at low cost.