The quantification of sulfonated polyacrylamide (PAM-AMPS) by size exclusionchromatography with ultraviolet detection (SEC-UV) is widely used to monitorenhanced oil recovery (EOR) processes and water disposal. However, the literaturedoes not provide information on how hydrolysis degradation during the productionprocess can affect the method for monitoring polymer concentration. Thus, thiswork aims to evaluate the effect of hydrolysis degradation of PAM-AMPS on itsquantification by SEC-UV. For this purpose, an accelerated controlled hydrolysisdegradation method was developed. PAM-AMPS standards with different degreesof hydrolysis (HD = 7 – 33 percent) were obtained. Different methods for determiningHD (13C NMR, FTIR, and back titration) were evaluated and found to bestatistically equivalent. Quantification by SEC-UV showed that the method ssensitivity depends on the degree of hydrolysis. Errors ranged from 20 percent to 40 percent starting at 13 percent HD but were negligible below 10 percent HD. These values decreased with increasing polymer concentration. The results indicate two compensatoryfactors for the decrease in molar absorptivity due to the substitution of amide bycarboxylate: the sulfonate group acting as an additional chromophore and a red shiftin the absorption spectra due to hydrogen bonding at higher concentrations. Thus,this study demonstrates that increasing the concentration of this type of sulfonatedpolyacrylamide can reduce quantification errors by minimizing the effect of HD incomplex aqueous samples subject to chemical degradation, highlighting theimportance of determining the degree of hydrolysis for accurate PAM-AMPSquantification by SEC-UV.