The objective of this dissertation was to investigate the pedagogical practices of university professors from four Chemistry s teaching courses, located in the state of Rio de Janeiro, seeking a theory-practice articulation. It is recognized that these teacher s preparation courses were historically marked by a technicist model that caused dichotomies between theory and practice and between specific and pedagogical knowledge which are perceived to this day. There was a search for teaching practices that distanced themselves from these technical and/or content models and presented an innovative character when they brought new possibilities of theory-practice articulation to Chemistry s teaching courses. The methodology was built in two stages: application of an online questionnaire to graduate students and semi-structured interviews with university professors indicated by the students in the first stage of the research. The main theoretical references were the studies by Candau and Lelis, Nóvoa, Gatti and André. The data produced were treated using content analysis according to Bardin. The results revealed that the appointed university professors use multiple teaching strategies to achieve their educational goals. Among the objectives, the following stand out: the human dimension of education, the contextualization of Chemistry contents and the importance of the teacher being also a researcher. The effect of pedagogical traditions and disciplinary traditions was also perceived as obstacles to the innovation of pedagogical practices in the preparation of new Chemistry teachers. It is concluded that university professors balance themselves between traditional and innovative practices, as they promote moments of collective work, curriculum articulation and approximation with the school, which are fundamentals for the theory-practice articulation.