Issues related to cultural differences intrude the daily school life mainly by pressures made by movements that legitimate their knowledge and fight for status in the school curriculum. While having the social rule to produce its own knowledge, mainly through teacher s practice in classroom, the school can not be seen as a simple pre-existing knowledge transmitter. As a contending value, the acknowledgement of sexual diversity consist as a controversial debate within the school, even in science and biology, disciplines traditionally charged with analyzing the aspects of human body functioning and sexual intercourse. This study aimed to understand how science and biology teachers, whose discussion of sexual diversity goes beyond the hegemonic heterosexuality standards, conceive this subject as part of the school curriculum. For this, semi-structured interviews were conducted with ten teachers and their speech were analyzed. We assess the findings of the interviews with three theoretical perspectives: (i) the school knowledge, recognizing a unique structure constructed at school; (ii) teacher knowledge, to value the teaching role in the construction of these school knowledge; and (iii) sexual diversity as a social and historical analysis category. We conclude the study showing the potential of the concepts of school and teaching knowledge for the development of science teaching practices involving intercultural subjects, such as sexual diversity.